Relation of rumen ATP concentration to bacterial and protozoal numbers

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Relation of rumen ATP concentration to bacterial and protozoal numbers.

Cultures of Streptococcus bovis and mixed populations of rumen bacteria were used to investigate the concentration of ATP and rumen bacterial numbers at various stages of growth. ATP, extracted with Tris buffer, was analyzed using the firefly luciferin-luciferase bioluminescent reaction. ATP concentrations of S. bovis and mixed cultures of rumen bacteria significantly correlated with live cell ...

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Reponses of sheep to a vaccination of entodinial or mixed rumen protozoal antigens to reduce rumen protozoal numbers.

Two rumen protozoa vaccine formulations containing either whole fixed Entodinium or mixed rumen protozoa cells were tested on Merino sheep with the aim of decreasing the number and/or activity of protozoa in the rumen. Negative control (no antigen) and positive control (Tetrahymena corlissi antigens) treatments were also included in the experiment. Blood and saliva were sampled to measure the s...

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Insecticides containing organophosphate, chlorinated hydrocarbon, and carbamate were tested with bovine ruminal ingesta fractions. Rumen bacteria exposed to insecticide levels of 0 to 500 ppm in rumen fluid for 4 hr were inoculated into rumen fluid-starch feed extract medium. No apparent significant bacterial count inhibitions were noted. Also, when insecticides were used as carbon sources at c...

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Ruminal bacteria and protozoa provide excellent organic phosphorus (P) sources for the host animal. This experiment was conducted to compare the bioavailability of bacterial and protozoal P in comparison with that of dicalcium phosphate (DCP) as a reference phosphorus source. Bacteria and protozoa were isolated from the rumen contents of dairy cows and the chick-model was used to determine the ...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1983

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.46.3.533-538.1983